Patton Oswalt

Uncle Ben (voice)

Jim Cummings

Hammerhead (voice)

Nadji Jeter

Miles Morales (voice) / Security Robot (voice)

Alastair Duncan

Adrian Toomes (voice) / Thug #3 (voice)

Robbie Daymond

Bully #1 / Hot Dog Announcer / Ticket Taker (voice) / Peter Parker / Spider-Man (voice) / Cop #1 (voice)

John DiMaggio

Policeman (voice) / Security Guard/Customer (voice) / Raymond Warren (voice)

Nancy Linari

Bomb Voice (voice) / Landlady (voice) / Woman Bystander (voice) / Aunt May (voice)

Max Mittelman

Harry Osborn (voice) / Police Officer #1/Osborn Technician #2/Police Officer #2 (voice) / Male Clerk/Guy in Line (voice) / Thug #2 / Little Kid (voice)

Melanie Minichino

Anya Corazon (voice) / Chaperone (voice) / Female Clerk (voice) / Helicopter Pilot (voice) / Student #1 (voice)

Ben Diskin

Flash Thompson / Student #2 (voice) / Flash Thompson (voice) / Police Officer #2/Hoodlum #1/Police Officer #4 (voice) / Osborn Recruiter #1 (voice)

Scott Menville

The Living Brain / Cop #2 (voice) / Doc Ock / Otto Octavius (voice) / Grady Scraps (voice)

Josh Keaton

Norman Osborn (voice) / Hologram (voice) / Unidentified Voice / Police Officer (voice) / Prison Guard (voice)